Supreme Pharmacist System Chapter 16: Perfect PCR experiment
Wu Yan knew that Zhou Wen must have a special extraction technique.
But seeing that he didn't want to say it, she didn't force it. After all, this is someone else's housekeeping skills, and it's harmful to leak it out.
Then the two talked about their treatment. Wu Yan repeated what she said to Shi Lei before to Zhou Wen, and finally asked with a smile, "Do you have any questions?"
Zhou Wen thought of the task and said, "Can I ask if I am a contract worker or a regular worker?"
"Well..." Wu Yan hesitated for a while, she didn't expect Zhou Wen to ask this question.
She believes that Zhou Wen should be very clear about the current situation of biology students. The contract of Jiangzhou First People's Hospital is so generous, not to mention that he is a small undergraduate student, even if he is a master student and doctoral student in biology. , a lot of people want it.
He actually wanted to go to the sky in one step and directly solve the problem of staffing. It was really too much. She herself is still a contract worker.
Wu Yan thought about it and said, "Generally speaking, if you are hired by the hospital, it must be a regular worker, but you are a contract worker for the PCR team of the testing center, so I am afraid it can only be a contract worker." /
"Contract workers..." Zhou Wen hesitated.
In fact, contract workers are still regular workers. He really doesn't care. As long as he can go to the testing center to practice and practice, he is willing to be a temporary worker.
This is not a task that requires him to become a regular worker within a week. He can't help but stretch.
For 1000 experience points and 1000 points, there is also a chance to open a black iron box.
Wu Yan originally thought that the matter was done, but she didn't expect it to happen again, so she said bitterly: "Although you are a contract worker, the salary is calculated according to the regular worker, and there is no difference.
In addition, everything happens step by step. You go to work first, and it is not too late to solve the problem of preparation after a while. "
Zhou Wen smiled and asked: "You are my senior sister, and you have also been in the hospital for a year. To be honest, if I really signed a contract worker, when will the establishment be resolved?"
"Well..." Wu Yan was a little embarrassed, and raised her coffee cup to cover it up.
After putting down the cup, Wu Yan said, "That's it, I'll help you go back and ask. But to be honest, don't have high expectations."
Zhou Wen nodded and said with a smile: "Okay, then I will trouble Senior Sister."
"You're welcome."
Zhou Wen was still ecstatic, so he didn't stay too long. He got up and left after a few polite words.
…
When he returned to the laboratory, Yang Yudong and Gu Hongyun couldn't wait to ask him if he was successful in the interview?
Zhou Wen smiled and said, "I made a condition. She said she would go back to discuss with the leader, and then give me a letter of approval after the discussion."
Yang Yudong said speechlessly: "The conditions are so good, do you still need to put forward conditions?"
Zhou Wen said: "I just want to try their reserve price because of the rare opportunity."
Yang Yudong: "..."
He really wanted to use the extraction gun to take out a little bit of Zhou Wen's brain and see what was going on in his mind all day long?
Zhou Wen smiled and started the PCR experiment.
The full name of PCR is PolymeraseChain.
The Chinese name is "polymerase chain reaction", also known as "polymerase chain reaction", which is an in vitro nucleic acid amplification technology developed in the mid-1980s.
The target gene or a DNA fragment to be studied can be amplified to 100,000 or even a million times within a few hours in a test tube.
The principle is basically similar to DNA replication in human cells, but the reaction system is relatively simple.
Of course, that doesn't mean PCR is easy.
In contrast, PCR is very difficult.
First, through high temperature "denaturation", a piece of double-stranded DNA is melted into two single-stranded DNA.
After "annealing", at low temperature, the "primer" and the DNA as a template are complementary to form a local double-stranded.
Finally, the medium-fire "extension", through the DNA polymerase catalyzed by the primer as the starting point of the DNA chain to produce an extension reaction, into two double-stranded DNA.
This process is repeated over and over again.
1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...
Through PCR technology, a sufficient amount of DNA can be amplified from a hair, a drop of blood, or even a cell for analysis, research and detection and identification.
Such as paternity test;
Such as vaccine research;
Such as virus disease prevention;
Or genetic disease research, etc., need a lot of PCR.
Of course, in the rice aphid biochemical reagent experiment Zhou Wen did today, it was actually unnecessary to do PCR.
Aphid is a very low-level insect, and it is generally used directly on it with drugs, and then it is enough to see its genetic characteristics.
The reason why we need to do PCR is to learn PCR.
The PCR reaction system includes water, buffer, DNTP (deoxyribonucleoside triphosphate), primer P1/P2/DNA template and TAQ enzyme.
After everything was ready, Zhou Wen adjusted the range of the extraction gun, and then extracted 30 μl of water into three sets of PCR tubes.
Strictly follow the operating procedures when sucking liquid, **** liquid vertically, **** slowly and release slowly.
After changing the pipette tip, 5 ml of buffer was extracted into the PCR tube.
Add DNTP/P1/P2/DNA template to the tube separately...
Because of this period of study, except for being a little nervous at the beginning, Zhou Wen did it in an orderly manner.
After the required raw materials are added, the next step is centrifugation.
After setting the speed as required, wait for three minutes and the centrifugation is completed.
Zhou Wen took out the PCR tube containing the mixture from the centrifuge and immediately put it on the PCR machine for amplification.
Set the pre-denaturation at 94°C for 4min.
Then set a 20-cycle amplification stage.
High temperature, low temperature, medium temperature, high temperature...
1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...
Zhou Wen waited anxiously, feeling like a husband outside the delivery room, silently praying "the mother and child are safe" in his heart, and at the same time wondering, will he be born a boy or a girl? Are you healthy or not?
At this moment, Zhou Wen suddenly thought that system points could speed up the progress of the experiment. Instead of waiting here for a few hours, why not take a look?
No one here sees it anyway.
Thinking of this, Zhou Wen immediately summoned the personal attribute page. After clicking the points, an option popped up in front of him.
[Would you like to spend points to speed up the progress of the experiment? Yes/No]
[Yes].
[A total of 20 points will be consumed for this task, please confirm again]
Zhou Wenjian only needs 20 points to speed up the progress of the experiment, so there is no reason to hesitate, and he immediately chooses to confirm.
Before Zhou Wen could react, the next second, the PCR machine had already completed the amplification.
Zhou Wen was astonished, the power of the system was simply mysterious.
He sighed in his heart, and soon he became nervous again.
This is the first time to do PCR. I wonder if it will be successful?
Zhou Wen turned on the PCR machine and took out the PCR tube.
Originally, the following is the product analysis, but Zhou Wen has the eyes of true vision, narrowed his eyes slightly, and soon he saw the high-density double-helix structure of DNA.
“perfect~”
If it wasn't for wearing a mask, Zhou Wen would have laughed out loud.
One person who studies biology counts as one. Who didn't learn to do PCR after countless failures. Who can be like him, doing amplification for the first time and succeeding in the first time?
And the amplification is surprisingly good, just perfect.
…
Jiangzhou City, First People's Hospital ~IndoMTL.com~ Testing Center, PCR group.
Wu Yan returned to the hospital after having lunch outside, and immediately reported the situation to the team leader's office.
"Where are the people?" Zhang Shuguang, who was writing the experiment report, asked without looking up.
Wu Yan said a little embarrassedly: "That... hasn't come yet."
When she went, she patted her chest and assured her that she would be caught, but the cows were blown up.
Zhang Shuguang raised his head and said unexpectedly, "Why, you don't want to come?"
Wu Yan shook her head and said, "No, he just said..."
Wu Yan explained Zhou Wen's request to Zhang Shuguang.
Zhang Shuguang tapped on the desk with his left hand holding the pen, and said with a smile: "You say you, such a smart person, how can you lose the chain at a critical time?"
Wu Yan asked strangely: "Why did I lose the chain? He wants to be paid as a regular worker. How can I do it for you?"
Zhang Shuguang said with a smile: "What's the matter? He is a biology undergraduate student, and he dares to open up to you to ask for a career plan. Don't you think about it?"
"Why?" Wu Yan wondered, she quickly reacted, and said in surprise, "Oh~~~ you mean..."
Zhang Shuguang laughed and said, "You've already come to look for others. As long as it's not too outrageous, you should promise him first. If you have any questions, you can talk about it later. Verbal promises are not guaranteed, right?"
"Mmmm~ Boss, I know I'm wrong." Wu Yan nodded again and again, admitting her mistake, "Then I'll call him now?"
Zhang Shuguang shook his head amusingly, "We are back, so what's the hurry, I'm afraid that he won't get away. Anyway, we are also the first hospital in Jiangzhou, so we shouldn't be too underestimated. Let's stretch him for two days before we talk about it." /