Supreme Pharmacist System Chapter 190: Please don’t take me with you bragging
No. 2333, Jiangzhou Science and Technology Park, Institute of Cryptococcosis.
It has been more than half a year since its establishment last year.
But so far, the Institute has found nothing.
The reason has also been said before, it is too difficult to clone cells, because the content of antigenic B cells in serum is too low.
After the B cells are isolated, the sensitivity and specificity of their sequence amplification and determination are also very high.
Even if the sequence is amplified and the antibody is expressed, the "neutralizing effect" of the antibody needs further identification.
In short, it is extremely difficult to reproduce antigenic B cells.
Of course, the above is for the average person.
Does not include Zhou Wen.
He has now upgraded several disciplines to LV4, and he can almost start trying to do this task.
This mission brought Zhou Wen not only experience, but also other additional things, such as influence in academia, which other missions could not bring him.
On the afternoon of May 5, Zhou Wen officially started cell cloning research.
The first step is to isolate B cells.
This is extremely difficult for others, but for Zhou Wen, who has true vision, it is a piece of cake.
First of all, his eyes can directly see the antigenic B cells in the serum;
Secondly, the upgrade of biological immunology to LV4 brought him not only pure knowledge, but also a deeper understanding of antigenic B cells, and he mastered a unique set of separation methods.
And these techniques are independent of existing biological knowledge.
One week later, Zhou Wen successfully isolated the B cells of cryptococcosis antigenicity.
Li Feng and Fan Bo, who had always criticized Zhou Wen in their hearts, almost didn't open their eyes in shock.
The other 13 doctoral researchers in the lab were also shocked.
Everyone's admiration for Zhou Wen is like a surging river, endless, and like the flood of the Yellow River, out of control.
But no one cheered!
This is just the first step in the Long March.
Next is the highlight - "Sequence Amplification"!
This is also difficult for ordinary people, because antigenic B cells are too rare, and the failure rate is as high as 99% during sequence amplification!
And if you fail, you may never have the chance again.
But this is not too big of a problem for Zhou Wen.
Don't forget, what did Zhou Wen start with, DNA extraction and amplification!
The world extracts DNA and PCR, he considers himself the second, and no one dares to claim the first.
Of course, Zhou Wen didn't dare to take it lightly, so he cheered up and went all out.
Because there are too few specimens.
If it fails, the isolated B cells will be discarded, and it will take a week to re-isolate, and it may not be a one-time success!
It is similar to the mouse DNA amplification procedure, except that he has redesigned the primers.
Multiple simulation experiments were also conducted.
Six days later, Zhou Wen successfully expanded enough antigenic B cells, and after product analysis, it was determined that the expansion was successful!
There is no shortage of research samples anymore.
The whole lab is boiling!
Everyone looked at Zhou Wen like a god.
If one-time isolation of antigenic B cells still belongs to the category of human beings, then the one-time expansion of antigenic B cells without any operating experience and no chance of failure is completely non-human technology. .
At this moment, Zhou Wen has become their spiritual belief!
Even if he says he is an alien now, they still believe it!
Next is the antibody expression assay.
Antibody expression is a multi-step process that begins with transcription from DNA to mRNA and culminates when mRNA is translated into protein...
The 30,000-word introduction is omitted here.
Not surprisingly, the sensitivity of antibody expression is naturally a one-time pass.
Li Feng and a bunch of other doctors were not surprised at all, and even took it for granted.
The next step is the most important and final step of the antibody "neutralization effect" test.
Because the "antibody" itself only has the effect of recognizing the target antigen, and does not have the effect of killing or rejecting the target antigen.
And the "neutralization effect" is that the antibody binds to the antigen to produce a precipitate or cell cluster, which is then phagocytosed by the phagocytic cells, thereby killing the antigen!
If the neutralization test is successful, it means that the cryptococcosis vaccine has been successfully manufactured.
……
Xiangya Hospital, Director's Office of the Department of Infectious Diseases.
Cheng Yanqing was writing an experiment report when the phone on the desk rang.
She picked it up and looked at it. It was Pan Renfeng, a member of the Virus Exchange Home and the director of the Department of Infectious Diseases of Harmony Hospital.
"What's the matter, Director Pan?"
"Zhou Wen said that the antigenic B cells they expanded have been tested for antibody expression, and the neutralization effect test will be carried out soon."
Cheng Yanqing was stunned for a moment, then said in shock: "What did you say?"
"I said that Zhou Wen and the others have isolated and expanded the antigenic B cells of cryptococcosis, and the neutralization effect test will be carried out next."
"Impossible!" Cheng Yanqing said decisively, "I called the research institute at the beginning of the month, and basic research is still being done there. It is impossible."
Pan Renfeng said: "This is not what I said, this is what Zhou Wen said, you can take a look in the group."
When Cheng Yanqing heard the words, he immediately opened the WeChat group, and he really saw Zhou Wen's speech.
It was Xia's old house who asked Zhou Wen what he was doing recently, and Zhou Wen casually replied that he was cloning the antigenic B cells of cryptococcosis.
Then Xia's old house asked him how his research was going?
Zhou Wen said that the neutralization effect test will be started immediately.
Zhou Wen's words blew up all the big guys in the group who were diving and peeping at the screen.
Everyone couldn't believe it.
But after Zhou Wen posted a few test data sheets, the group was suddenly fried.
Cheng Yanqing clicked on the test data and looked at it, and was shocked beyond measure!
She wonders if today is April Fool's Day?
How could such a thing possibly exist?
She clearly called Li Feng at the beginning of tomorrow, and he sighed and said that there was no progress, and his words revealed his complaints against Zhou Wen.
But now...
Cheng Yanqing immediately called Zhou Wen, who was on the phone there.
Cheng Yanqing did not hang up, but waited.
About tens of seconds later, the call was connected.
"Zhou Wen, what's going on?"
"That... I can't explain it for a while. So, if you have time, Director Cheng, come to Jiangzhou tomorrow and have a face-to-face interview."
Cheng Yanqing had too many things to ask in his heart, but at this moment he didn't know where to start, so he had to hang up temporarily.
How could Yan Qing wait until tomorrow if you don't have the process?
Without saying a word, I immediately set off for Jiangzhou.
Called Li Feng, the head of the laboratory, over and over again on the way.
Finally got through.
Under Li Feng's narration, Cheng Yanqing's jaw almost didn't fall, and he couldn't recover for a long time.
"Dududu---"
Finally, Cheng Yanqing was awakened by the sound of the horn behind him, and only then did he realize that he had stopped at the traffic light intersection.
Cheng Yanqing took a deep breath after starting and asked, "Why don't you tell me about such a big thing?"
Li Feng said: "Because Zhou said, the process is not important, but the result, if the neutralization effect test fails, all the previous sweat and efforts are meaningless, so there is no need to let everyone know. "
Cheng Yanqing: "..."
Is this human?
Successful isolation of antigenic B cells is an amazing feat, and it also has a very important reference for the isolation of antigenic B cells in other rare diseases!
In her opinion, this technology alone is worth three lancets!
How can you say it's pointless?
As for the subsequent sequence amplification, it is also very important.
"This little guy is so outrageous!"
Cheng Yanqing said something and couldn't help laughing.
……
Sunday, May 26, 2019.
At 9:30 in the morning, Jiangzhou Institute of Cryptococcosis conducted a public test on the neutralization effect of antigenic B cells of cryptococcosis.
Some good friends and leaders familiar with Zhou Wen visited the site.
Like Pan Renfeng, Cheng Yanqing, Gu Yunwen, Mu Yuanqing, Lin Huaidong, Zhang Wen, as well as several leaders of Jiangzhou Medical College and highly respected experts and professors.
The results were not surprising.
Under the public's attention, the cell group produced by the combination of the antibody of cryptococcosis and the antigen of cryptococcosis was phagocytosed by phagocytes little by little!
"Clap clap-"
In the observation room outside the laboratory, there was warm applause!
"Congratulations Director Cheng, you guys are amazing."
"This is an amazing invention! Director Cheng, you are great!"
“…”
Faced with the praise from a group of old friends, Cheng Yanqing said with a good laugh: "It's alright, it's alright, you don't have to brag here! I'm also the leader, all the research work is done by Zhou Wen. I don't care."
The experts at the scene knew what was going on, so everyone laughed.
It's okay to joke around with friends, but they're not that thick-skinned when it comes to ripping off other people's academic achievements!
And this one can't be robbed at all.
Several core technical processes in it, if the experimental recovery cannot be done by then, it will be thrown into the grandmother's house as a shame.
Experts are sorry, but one of the leaders of Jiangzhou Medical College laughed and said, "Director Cheng is too modest!
It is undeniable that Xiao Zhou's outstanding ability is undeniable, but we can't ignore the role of leadership. Without the leadership's instructions and proper scheduling, their young people can only turn around like flies without heads, right? "
Cheng Yanqing was really embarrassed to see this leader's serious face~IndoMTL.com~, and said to herself: "I'm not humble, please don't take me with you!"
Seeing the expression on Cheng Yanqing's face, Pan Renfeng, Lin Huaidong, Zhang Wen and others couldn't help laughing.
As for the potbellied leader of Jiangzhou Medical College, he was inexplicable and said to himself, "Are my words funny?"
But he didn't dare to question, let alone show his face.
Pan Renfeng, Gu Yunwen, Lin Huaidong, Zhang Wen, etc., which one is not a big shot in the domestic medical system?
That's a direct conversation with the head above.
At his level, it's not enough to see...
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