Due to the particularity of prions, Zhou Wen was extra careful when conducting experiments.

Biological masks, biological gloves, biological protective clothing, fully armed.

The four of them, Qin Haitao, were just helping out, and they didn't directly touch the vessel containing the virus.

There are currently four methods for detecting prions.

1. Electron microscopy.

2. Capillary SDS-gel electrophoresis.

3. Capillary electrophoresis immunoassay of fluorescently labeled peptide chains.

4. Two-color strong fluorescence target scanning method.

The second "gel electrophoresis" is the best and most direct detection method.

PrPsc is purified mainly by physical and chemical methods-differential speed, ultracentrifugation, sodiumN-(lauroyl sarcosine), PK treatment.

Then perform capillary SDS-gel electrophoresis on a "capillary electrophoresis apparatus".

According to the time and peak shape of the absorption peak at the wavelength of 214nm when each component passes through the detector, the prion pathogenic factor PrPsc is identified.

This method requires less specimen and does not require immunological reagents, so the judgment is very intuitive.

The disadvantage is that two kinds of equipment, "ultracentrifuge" and "special capillary electrophoresis instrument" are required.

Ultracentrifuges are basically purchased from foreign brands at present, with prices starting at 500,000 RMB.

The special capillary electrophoresis instrument for prions is also very expensive, requiring more than 200,000 RMB.

Even if it is expensive, there are few opportunities for the key to be used.

General hospitals can't even make money from purchasing equipment, and only a wealthy tertiary hospital like Jiangzhou Hospital will buy it.

Because of this, medical resources in big cities are scarce.

The small city has a strange disease, and you can't even get tested.

According to physical and chemical methods, Zhou Wen carefully prepared reagents step by step, extracted diluents, and placed them on an ultracentrifuge to separate mutant prion proteins.

The newly upgraded biological immunology makes him even more handy.

During this process, Zhou Wen was also teaching Qin Haitao and Dong Wenying about the things to pay attention to in the routine physical and chemical tests of prions.

"When adding lauroyl, you must remember..."

These experiences, not to mention university professors, even experts from domestic prion research institutes can't explain them clearly.

Because domestic research on viruses, especially prions, is still in its infancy, and only those top foreign research institutes can master these practical experiences.

Such as the Yeast Prion Institute at the University of Kent, UK.

For a long time, new discoveries in the field of prion proteins have been "monopolized" by several research teams in the field of yeast.

Those top research institutes will not easily teach you technology.

Unless you can get their offer.

And Qin Haitao and the others obviously didn't realize how lucky they were, but they felt that Zhou Wen's explanation was very simple and easy to understand, and even told them the essentials of the action.

When Zhou Wen spoke, Qin Haitao and Dong Wenying both listened attentively.

As for the other two old experimenters, although they were listening on the surface, they didn't actually listen at all.

Because they felt that it was impossible to extract prions with Zhou Wen's method, and such teaching was simply on paper.

Zhou Wen doesn't have an "electric eye". How could he know these specific experimental variables? Everything is just his guess.

After PrPsc was purified, Zhou Wen injected the prion-containing supernatant into the Beckman P/ACE5500 for electrophoresis experiments.

"Okay~" Zhou Wen breathed a sigh of relief after the injection was completed.

"Qin Haitao, please stay and take care of the machine, while others wash their hands and eat."

Everyone went to the sink to wash their hands after hearing the news...

After eating, Zhou Wen didn't dare to go anywhere, and went straight back to the laboratory.

The samples obtained by the conventional extraction method are too rare, and I don't feel relieved if I don't see it in person...

At half past seven in the evening, the electrophoresis experiment ended.

One of the experimenters, Huang Xingzhong, casually glanced at the electrophoresis analysis sheet, subconsciously thinking that this experiment would fail as before.

However, the next second, a surprised look appeared on his face, "Hey, come and see, it seems that prions have been detected..."

When the rest of the people heard the words, they gathered around.

"Hey, it's really alright."

"Teacher, come and see, we have detected prions."

"Hiss... it shouldn't be, how is this possible, is it a mistake..."

Zhou Wen took the analysis sheet and looked at it carefully, and soon his face showed an uncontrollable joy.

No matter how well the previous experiment went, nothing is 100% until the results are in.

For example, a malfunction of the capillary electrophoresis instrument can also cause the detection to fail.

This will make all previous efforts in vain.

So sometimes doing experiments, luck also accounts for a part of the factor.

After confirming that it was not dazzling, everyone's face showed indescribable excitement.

To be honest, including Qin Haitao and Dong Wenying, they do not think that prion protein can be extracted by conventional methods.

Under the premise of no hope, it is really a proud thing to rely on conventional collection methods to extract prion protein.

After the excitement was over, Qin Haitao immediately informed Yang Liqun and Zhang Shuguang.

After the two heard the words, they rushed over immediately.

"According to testing, this is an aggregated prion..."

Yang Liqun and Zhang Shuguang looked at the plain electrophoresis analysis sheet, their faces were full of surprise, joy, excitement, excitement and disbelief.

"Is this really detected in urine?"

"It's the Dezhang group! The samples were collected by Zhou Wen and I."

Hearing Dong Wenying's words, Yang Liqun and Zhang Shuguang looked incredulous.

In the case of routine detection and unconventional detection, the extraction of prions has completely different meanings.

This is a very important discovery with high research value.

"You are all good. Although this is only a small step in prion research, it is a big step for China in the field of prions."

"Thank you for the praise from Team Yang. It's mainly because of Teacher Zhou. If he hadn't been persistent, we might have given up at first sight."

Yang Liqun and Zhang Shuguang looked at Zhou Wen.

Both of them have always been very optimistic about this young man, and Zhou Wen lived up to his expectations, showing a very strong talent in biomedicine.

And often surprises them.

Like this time.

Yesterday they also predicted that it is absolutely impossible to extract prion protein under conventional collection methods.

As a result, Zhou Wen "slapped his face" the next day.

But this face is comfortable.

If they can, they want him to keep punching in the face, preferably with a bow left and right!

After being excited, Yang Liqun and Zhang Shuguang went to the computer to analyze and study the sample collection records, hoping to find certain patterns.

Zhou Wen also admires Yang Liqun and Zhang Shuguang for their actions.

This is the spirit of scientific research.

Although no one wants to collect prion proteins in a routine state, they cannot stop researching because they don't want to.

Only knowing which factors lead to the excretion of prion proteins through body fluids can better prevent and control.

The next morning, Yang Liqun reported Zhou Wen's findings to the hospital, requesting the official establishment of the "prion research team ~IndoMTL.com~ hospital leaders under the testing center. A special meeting was held to discuss whether to approve or not. Form this research group?

As the First People's Hospital affiliated to Jiangzhou University, it is not too bad in terms of hardware equipment or talent pool, but why can't it compare to other hospitals?

The main reason is that the Jiangzhou No. 1 hospital has everything and everything, and there is no "unique skill" that can be mastered.

For example, the Jinling Army General Hospital in the province is well-known in the country in terms of kidney disease.

For example, Huashan Hospital in Zhonghai is very good in dermatology, infectious diseases, and neurology.

Another example is the First Hospital of Kyoto University, which is invincible in urology.

And the Orthopedic Department of Changzheng Hospital...

As for the four celestial groups, North Union, South Xiangya, East Qilu, and West China, they are at the level of immortals, and Jiangzhou Hospital can't keep up.

In short, Jiangzhou Hospital can be compared with other municipal hospitals except Jinling in the province, but it is not worth mentioning in China.

Because of this, Jiangzhou Hospital urgently needs a medical technology "Zhenchangzi".

In the end, the research team's application was unanimously approved.

Because Jiangzhou City... It should be said that the entire Eastern Jiangsu Province has no authoritative experts on prions at all.

As the initiators of the virus research, Yang Liqun, Zhang Shuguang and Zhou Wen have undoubtedly become the backbone candidates of the research team.

Under the recommendation of Yang Liqun and Zhang Shuguang, Zhou Wen became the team leader of the [Prion Research Group] of the testing center, and the two of them are honorary deputy team leaders.

The first group members: Qin Haitao, Dong Wenying, Huang Xingzhong, Liu Shan...

.

——

ps: I'm sorry, please...

Due to the particularity of prions, Zhou Wen was extra careful when conducting experiments.

Biological masks, biological gloves, biological protective clothing, fully armed.

The four of them, Qin Haitao, were just helping out, and they didn't directly touch the vessel containing the virus.

There are currently four methods for detecting prions.

1. Electron microscopy.

2. Capillary SDS-gel electrophoresis.

3. Capillary electrophoresis immunoassay of fluorescently labeled peptide chains.

4. Two-color strong fluorescence target scanning method.

The second "gel electrophoresis" is the best and most direct detection method.

PrPsc is purified mainly by physical and chemical methods-differential speed, ultracentrifugation, sodiumN-(lauroyl sarcosine), PK treatment.

Then perform capillary SDS-gel electrophoresis on a "capillary electrophoresis apparatus".

According to the time and peak shape of the absorption peak at the wavelength of 214nm when each component passes through the detector, the prion pathogenic factor PrPsc is identified.

This method requires less specimen and does not require immunological reagents, so the judgment is very intuitive.

The disadvantage is that two kinds of equipment, "ultracentrifuge" and "special capillary electrophoresis instrument" are required.

Ultracentrifuges are basically purchased from foreign brands at present, with prices starting at 500,000 RMB.

The special capillary electrophoresis instrument for prions is also very expensive, requiring more than 200,000 RMB.

Even if it is expensive, there are few opportunities for the key to be used.

General hospitals can't even make money from purchasing equipment, and only a wealthy tertiary hospital like Jiangzhou Hospital will buy it.

Because of this, medical resources in big cities are scarce.

The small city has a strange disease, and you can't even get tested.

According to physical and chemical methods, Zhou Wen carefully prepared reagents step by step, extracted diluents, and placed them on an ultracentrifuge to separate mutant prion proteins.

The newly upgraded biological immunology makes him even more handy.

During this process, Zhou Wen was also teaching Qin Haitao and Dong Wenying about the things to pay attention to in the routine physical and chemical tests of prions.

"When adding lauroyl, you must remember..."

These experiences, not to mention university professors, even experts from domestic prion research institutes can't explain them clearly.

Because domestic research on viruses, especially prions, is still in its infancy, and only those top foreign research institutes can master these practical experiences.

Such as the Yeast Prion Institute at the University of Kent, UK.

For a long time, new discoveries in the field of prion proteins have been "monopolized" by several research teams in the field of yeast.

Those top research institutes will not easily teach you technology.

Unless you can get their offer.

And Qin Haitao and the others obviously didn't realize how lucky they were, but they felt that Zhou Wen's explanation was very simple and easy to understand, and even told them the essentials of the action.

When Zhou Wen spoke, Qin Haitao and Dong Wenying both listened attentively.

As for the other two old experimenters, although they were listening on the surface, they didn't actually listen at all.

Because they felt that it was impossible to extract prions with Zhou Wen's method, and such teaching was simply on paper.

Zhou Wen doesn't have an "electric eye". How could he know these specific experimental variables? Everything is just his guess.

After PrPsc was purified, Zhou Wen injected the prion-containing supernatant into the Beckman P/ACE5500 for electrophoresis experiments.

"Okay~" Zhou Wen breathed a sigh of relief after the injection was completed.

"Qin Haitao, please stay and take care of the machine, while others wash their hands and eat."

Everyone went to the sink to wash their hands after hearing the news...

After eating, Zhou Wen didn't dare to go anywhere, and went straight back to the laboratory.

The samples obtained by the conventional extraction method are too rare, and I don't feel relieved if I don't see it in person...

At half past seven in the evening, the electrophoresis experiment ended.

One of the experimenters, Huang Xingzhong, casually glanced at the electrophoresis analysis sheet, subconsciously thinking that this experiment would fail as before.

However, the next second, a surprised look appeared on his face, "Hey, come and see, it seems that prions have been detected..."

When the rest of the people heard the words, they gathered around.

"Hey, it's really alright."

"Teacher, come and see, we have detected prions."

"Hiss... it shouldn't be, how is this possible, is it a mistake..."

Zhou Wen took the analysis sheet and looked at it carefully, and soon his face showed an uncontrollable joy.

No matter how well the previous experiment went, nothing is 100% until the results are in.

For example, a malfunction of the capillary electrophoresis instrument can also cause the detection to fail.

This will make all previous efforts in vain.

So sometimes doing experiments, luck also accounts for a part of the factor.

After confirming that it was not dazzling, everyone's face showed indescribable excitement.

To be honest, including Qin Haitao and Dong Wenying, they do not think that prion protein can be extracted by conventional methods.

Under the premise of no hope, it is really a proud thing to rely on conventional collection methods to extract prion protein.

After the excitement was over, Qin Haitao immediately informed Yang Liqun and Zhang Shuguang.

After the two heard the words, they rushed over immediately.

"According to testing, this is an aggregated prion..."

Yang Liqun and Zhang Shuguang looked at the plain electrophoresis analysis sheet, their faces were full of surprise, joy, excitement, excitement and disbelief.

"Is this really detected in urine?"

"It's the Dezhang group! The samples were collected by Zhou Wen and I."

Hearing Dong Wenying's words, Yang Liqun and Zhang Shuguang looked incredulous.

In the case of routine detection and unconventional detection, the extraction of prions has completely different meanings.

This is a very important discovery with high research value.

"You are all good. Although this is only a small step in prion research, it is a big step for China in the field of prions."

"Thank you for the praise from Team Yang. It's mainly because of Teacher Zhou. If he hadn't been persistent, we might have given up at first sight."

Yang Liqun and Zhang Shuguang looked at Zhou Wen.

Both of them have always been very optimistic about this young man, and Zhou Wen lived up to his expectations, showing a very strong talent in biomedicine.

And often surprises them.

Like this time.

Yesterday they also predicted that it is absolutely impossible to extract prion protein under conventional collection methods.

As a result, Zhou Wen "slapped his face" the next day.

But this face is comfortable.

If they can, they want him to keep punching in the face, preferably with a bow left and right!

After being excited, Yang Liqun and Zhang Shuguang went to the computer to analyze and study the sample collection records, hoping to find certain patterns.

Zhou Wen also admires Yang Liqun and Zhang Shuguang for their actions.

This is the spirit of scientific research.

Although no one wants to collect prion proteins in a routine state, they cannot stop researching because they don't want to.

Only knowing which factors lead to the excretion of prion proteins through body fluids can better prevent and control.

The next morning, Yang Liqun reported Zhou Wen's findings to the hospital, requesting the official establishment of the "prion research team ~IndoMTL.com~ hospital leaders under the testing center. A special meeting was held to discuss whether to approve or not. Form this research group?

As the First People's Hospital affiliated to Jiangzhou University, it is not too bad in terms of hardware equipment or talent pool, but why can't it compare to other hospitals?

The main reason is that the Jiangzhou No. 1 hospital has everything and everything, and there is no "unique skill" that can be mastered.

For example, the Jinling Army General Hospital in the province is well-known in the country in terms of kidney disease.

For example, Huashan Hospital in Zhonghai is very good in dermatology, infectious diseases, and neurology.

Another example is the First Hospital of Kyoto University, which is invincible in urology.

And the Orthopedic Department of Changzheng Hospital...

As for the four celestial groups, North Union, South Xiangya, East Qilu, and West China, they are at the level of immortals, and Jiangzhou Hospital can't keep up.

In short, Jiangzhou Hospital can be compared with other municipal hospitals except Jinling in the province, but it is not worth mentioning in China.

Because of this, Jiangzhou Hospital urgently needs a medical technology "Zhenchangzi".

In the end, the research team's application was unanimously approved.

Because Jiangzhou City... It should be said that the entire Eastern Jiangsu Province has no authoritative experts on prions at all.

As the initiators of the virus research, Yang Liqun, Zhang Shuguang and Zhou Wen have undoubtedly become the backbone candidates of the research team.

Under the recommendation of Yang Liqun and Zhang Shuguang, Zhou Wen became the team leader of the [Prion Research Group] of the testing center, and the two of them are honorary deputy team leaders.

The first group members: Qin Haitao, Dong Wenying, Huang Xingzhong, Liu Shan...

.

——

ps: I'm sorry, please...